An approach toward developing mitochondrial artificial chromosome in rice

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660001
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: Tohoku University
• Principal Investigator: TORIYAMA Kinya 東北大学, (連合)農学研究科(研究院), 教授 (20183882)
• Co-Investigator(Renkei-kenkyūsha): KAZAMA Tomohiko 東北大学, 大学院農学研究科, 助教 (30431464)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 育種学 / 遺伝学 / 遺伝子 / バイオテクノロジー / 植物

Outline of Final Research Achievements

To establish transformation system of rice mitochondria, we aimed to develop a mitochondrial artificial chromosome. We employed a utilization of plasmid-like circle DNA, B1 plasmid, which is stably present in mitochondria of BT-type and LD-type cytoplasmic male sterile rice. We carried out cloning of LD-type B1 plasmid and inserted our originally designed GFP expression cassette. Finally we constructed a putative mitochondrial artificial chromosome containing GFP cassette using an E. coli-based mini-circle vector technology to test whether it work as an artificial chromosome in rice mitochondria.

Free Research Field

植物分子遺伝学