2016 Fiscal Year Final Research Report
Establishment of novel plant genome modification technology using bacterial Type4 Secretion System
| Project/Area Number |
25660007
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Science in genetics and breeding
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | 四型分泌系 / ジーンターゲティング / 遺伝子水平伝達 |
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| Outline of Final Research Achievements |
RP4 plasmid transfers itself into eukaryotic cells by a Type4 Secretion System (RP4-T4SS), which is encoded in the plasmid. We found that the transferred liner ssDNA re-circularized exactly in yeast, and based on this finding, we intended to establish a gene-targeting method in plants.
We could not improve its transfer efficiency to the level, which is sufficient for applying to gene-targeting. Instead, we found a plasmid, which transfers and re-circularized in plant cells by Ti plasmid encoded Type 4 Secretion System (VirB/D4-T4SS). By using the plasmid, we constructed targeting vectors and helper plasmids, which possessed genes for enhancing homologous recombination. In spite of combining these vectors and helper plasmids, we have not detected the gene-targeting phenomenon in plant cells.
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| Free Research Field |
ゲノム応用科学
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