2014 Fiscal Year Final Research Report
"Light-switch" for higher production of foreign proteins in plant chloroplasts
| Project/Area Number |
25660008
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Science in genetics and breeding
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| Research Institution | University of Shizuoka |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 有用物質生産 / 葉緑体工学 / 遺伝子発現制御 / 光 / 転写 |
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| Outline of Final Research Achievements |
Costs for production of therapeutic proteins in animal cells decline down to 1/100 when they are made in plants. However, such exogenous proteins frequently interfere with the growth of plants. Therefore, "light-switch" for turning on the expression of foreign genes is needed after plants have grown. “Drought-switch” was also tried to develop. The selectable suppression of transcription of psaA, Arabidopsis chloroplast genome-encoded gene for photosystem (PS) I reaction center protein, was applied to establishment of "light-switch". The optimal light wavelength was determined at 700 nm. Reporter genes, CBRluc for red color luminescence and CBG68luc for green one as provided as Chroma-Glo Luciferase (Promega) were employed. An evaluation protocol on the basis of transient expression was established. A system for turning on the gene expression by light exposure was designed with RNAi, as the psaA regulation was negative.
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| Free Research Field |
農学
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