2016 Fiscal Year Final Research Report
Improvement of recombinant protein stability in plants
Project/Area Number |
25660010
|
Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
Allocation Type | Multi-year Fund |
Research Field |
Science in genetics and breeding
|
Research Institution | National Institute of Advanced Industrial Science and Technology |
Principal Investigator |
Matsuo Kouki 国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (10358012)
|
Project Period (FY) |
2013-04-01 – 2017-03-31
|
Keywords | プロテアーゼインヒビター / アグロインフィルトレーション / 組換えタンパク質生産 / 一過性発現 |
Outline of Final Research Achievements |
Recombinant proteins produced in plant cells are frequently degraded by endogenous plant proteases. To avoid recombinant protein degradation in plant cells, co-expression of protease inhibitors with the recombinant proteins would be efficient. In this study, it was clear that some protease inhibitor could improve recombinant protein yield in Nicotiana benthamiana plants. In the present study, a simple transient gene expression system based on Agrobacterium-mediated transformation was developed . Vacuum infiltration was applied to leaf discs from Nicotiana benthamiana plants with Agrobacterium suspension solution under conventional vacuum conditions in a needleless plastic syringe. This method can be conducted without specialized apparatuses or large amounts of Agrobacterium suspension solutions; thus, the simultaneous evaluation of multiple vectors will be easily possible.
|
Free Research Field |
分子生物学
|