2014 Fiscal Year Final Research Report
Analyses of virus elimination from asparagus seedlings by ascorbic acid and slow-freezing treatments
Project/Area Number |
25660019
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Horticultural science
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Research Institution | Hokkaido University |
Principal Investigator |
SUZUKI TAKASHI 北海道大学, (連合)農学研究科(研究院), 准教授 (30196836)
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Co-Investigator(Kenkyū-buntansha) |
SHIMURA Hanako 北海道大学, 大学院農学研究院, 助教 (20507230)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Keywords | アスパラガス / ウイルスフリー化 / アスコルビン酸 / 凍結処理 |
Outline of Final Research Achievements |
Asparagus virus 1 (AV-1) and Asparagus virus 2 (AV-2) have been considered as major pathogenic viruses that cause the reduction of productivity of asparagus. To prepare the virus-free asparagus in vitro culture for field production, we here tried to establish a new approach for virus elimination from asparagus by using ascorbic acid treatment and slow-freezing cryotherapy. Virus elimination was observed in seedlings treated by either ascorbic acid or slow-freezing, but there were differences in elimination rate among asparagus cultivars. In addition, enhanced growth of asparagus seedling after slow-freezing treatment was observed in some tested plants. AV-2 was more difficult to be eliminated from seedlings than AV-1, probably due to systemic infection by AV-2 in asparagus. Additional immunohistochemical analysis confirmed that AV-2 was localized in the leaf primordia and vascular tissue around shoot meristem of asparagus seedlings.
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Free Research Field |
園芸学
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