Nuclease mechanism leading early senescence in mesophyll cells of petals

2015 Fiscal Year Final Research Report

• Project/Area Number: 25660032
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Horticultural science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Mochizuki Hiroko 国立研究開発法人農業・食品産業技術総合研究機構, 野菜花き研究部門花き生産流通領域, 上級研究員 (60450318)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: チューリップ / ユリ / 花弁 / 老化 / ヌクレアーゼ / EST / マイクロアレイ

Outline of Final Research Achievements

In petals (inner tepals) of tulip and lily, we found signs of PCD, including DNA degradation and decreased protein content, earlier in mesophyll cells than in epidermal cells. we have generated an expressed sequence tag (EST) database by using a tulip cultivar ‘Ile de France’. From 64,922 contigs, we found at least 65 nuclease homologous. Based on the EST database, we arranged probes and examined a microarray analysis. Putative nuclease-associated genes were selected from the results of microarray analysis, which were up-regulated early in mesophyll cells. In lily petals (inner tepals), transcript level of S1/P1 type nuclease homolog was higher in mesophyll cells than epidermal cells after anthesis.

Free Research Field

園芸学