Insect taste receptors which can detect more than 5 basic taste of mammals

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660040
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: RYOICHI Sato 東京農工大学, (連合)農学研究科(研究院), 教授 (30235428)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 味受容体 / カイコ / BmGr

Outline of Final Research Achievements

Host plant recognition-concerning taste detection system was attempted to be cleared. First, a member of taste receptors, BmGrs which is expressing in a taste sensing cell of epipharyngeal sensillum in the labrum of Bombyx mori larvae was attempted to be cleared. A neural cell cluster of epipharyngeal sensillum was obtained using glass-capillary and single cell was dissected from it by laser capture microdissection (LCM). RT-PCT suggested that this cell expressed 6 kinds of BmGrs including BmGr10 and they were considered to construct inositol receiving system. Ca2+ indicators, GCaMP co-expressing system and Xenopus oocyte expressing system connected to two-electrode voltage clamp were used to determine the ligand of BmGr10. From these, inositol was demonstrated to be a ligand of BmGr10.

Free Research Field

昆虫生理学，昆虫病理学