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2014 Fiscal Year Final Research Report

Genomic characterization of the fungus-endobacterium symbiotic system for the fungal transformation engineering

Research Project

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Project/Area Number 25660045
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Plant nutrition/Soil science
Research InstitutionIbaraki University

Principal Investigator

OHTA Hiroyuki  茨城大学, 農学部, 教授 (80168947)

Co-Investigator(Kenkyū-buntansha) NARISAWA Kazuhiko  茨城大学, 農学部, 教授 (90431650)
SATO Yoshinori  独立行政法人国立文化財機構東京文化財研究所, 保存修復センター, 研究員 (50466645)
Research Collaborator FUJIMURA Reiko  茨城大学, 博士特別研究員
OHSHIMA Shoko  茨城大学, 大学院農学研究科
Project Period (FY) 2013-04-01 – 2015-03-31
Keywords応用微生物 / 菌類 / 細菌 / 生態学 / バイオテクノロジー
Outline of Final Research Achievements

The endobacterium harbored in the mycelium of a fungus Mortierella elongate 23-6 I-B1 was isolated as a pure culture (strain B1-EBT) by the draft genome information from the endobacterium fraction of the fungal cell homogenate. Morphological, biochemical, and phylogenetic analyses revealed the strain B1-EBT was not classified into known taxa. Therefore, we proposed a novel species in a novel genus, Mycophilus cysteinexigens, in the family Burkholderiaceae for strain B1-EBT. From the genome information, strain B1-EBT was found to lack several key genes responsible for cysteine biosynthesis as well as the glycolytic pathway. The strain possesses the type II secretion system but not chitin-degrading enzyme genes, a key factor in the bacterial invasion into the host cell. Therefore, it can be expected that the association of M. elongate with M. cysteinexigens is established through a new cell-to-cell interaction mechanism.

Free Research Field

環境毒性化学

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Published: 2016-09-02  

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