2014 Fiscal Year Final Research Report
Roles of autophagy during seed maturation in crop plants.
| Project/Area Number |
25660049
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Plant nutrition/Soil science
|
|---|
| Research Institution | Tokyo University of Science |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KURUSU Takamitsu 東京工科大学, 応用生物学部, 助教 (50422499)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Keywords | オートファジー / イネ / 脂質代謝 / 花粉形成 / 雄性不稔 / 種子登熟 / 葯 / タペート |
|---|
| Outline of Final Research Achievements |
We isolated several knockout mutants defective in ATG genes and found that autophagy is induced in postmeiotic tapetum cells specifically at the uninucleate stage during pollen development and is essential for postmeiotic anther development in rice. The mutant showed complete sporophytic male sterility, failed to accumulate lipidic and starch components in pollen grains at the flowering stage, showed reduced pollen germination activity, and had limited anther dehiscence. Lipidomic analyses suggested impairment of editing of phosphatidylcholines and lipid desaturation in the mutant during pollen maturation. In order to further analyze the possible involvement of autophagy during seed maturation, we attempted to construct transgenic plants applying a tapetum-specific promoter and an inducible promoter to express ATG genes in tapetum for complementation.
|
| Free Research Field |
植物分子生理学
|
