Investigation of Possibility and Environmental Adaptative Mechanism to Create a Resistance Strain for Quorum Sensing Inhibition

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660062
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Kyushu Institute of Technology
• Principal Investigator: MAEDA Toshinari 九州工業大学, 生命体工学研究科, 准教授 (00470592)
• Co-Investigator(Kenkyū-buntansha): MOROHOSHI Tomohiro 宇都宮大学, 工学研究院, 准教授 (90361360)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: クォーラムセンシング阻害 / 環境適応機能 / 耐性菌株 / 緑膿菌 / 代謝変換

Outline of Final Research Achievements

Inhibition of quorum sensing is a promising approach as a next-generation infection treatment unlike antibiotics which kill bacteria.
In this study, it was investigated if bacteria can have a certain ability to alleviate or avoid repression effect by quorum sensing inhibition. Therefore, a resistant strain to quorum sensing inhibition was screened under the condition in the presence of adenosine as a sole carbon source as utilization of adenosine is required by the function of quorum sensing. As a result, mexR and nalC genes in Pseudomonas aeruginosa were related to the resistance to quorum sensing inhibition and also Pseudomonas aeruginosa was able to biotransform a quorum sensing inhibitor.

Free Research Field

応用微生物学