Screening for new genes involved in antibiotics production from soil-metagenome using metatranscriptomic analysis

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660069
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: National Institute for Agro-Environmental Sciences
• Principal Investigator: FUJII Takeshi 独立行政法人農業環境技術研究所, その他部局等, 研究領域長 (00354100)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 放線菌 / 抗生物質 / キチン / 転写誘導 / メタトランスクリプトーム

Outline of Final Research Achievements

The applicant has found that the expression of antibiotic production-related genes of Streptomyces coelicolor growing in soil was activated by the addition of chitin. Based on this finding, in this study, the antibiotic production-related genes which expression is activated in soil after addition of chitin will be analyzed by sequencing RNA extracted from soil amended with chitin. The aim of this study is to get the genes that are involved in new antibiotics produced in soil by soil microorganisms.

Free Research Field

土壌微生物学