Construction of high-level production system for membrane protein using cyanobacteria

2015 Fiscal Year Final Research Report

• Project/Area Number: 25660075
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Ibaraki University
• Principal Investigator: Kimura Shigenobu 茨城大学, 工学部, 教授 (90291608)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 膜タンパク質 / 発現系 / シアノバクテリア / チラコイド / 膜移行シグナル

Outline of Final Research Achievements

Membrane proteins are very important in cell systems such as metabolism, substance transport, and signal transduction. High-level production system of recombinant membrane protein using easily caltivable bacteria is strongly required for the development of structural and functional studies on various membrane proteins. In this study, it was founded that recombinant protein could be accumulated in the thylakoid membrane fraction of a plant-type photosynthetic microorganism, cyanobacteria, using an N-terminal polypeptide containing signal sequence of the thylakoid membrane protein of the cyanobacteria.

Free Research Field

蛋白質工学