Regulatory mechanism of adipocyte differentiation by PLSCR3 mediated by extracellularly secreted vesicle exosomes

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660076
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Nagoya University
• Principal Investigator: MAKI Masatoshi 名古屋大学, 生命農学研究科, 教授 (40183610)
• Co-Investigator(Renkei-kenkyūsha): SHIBATA Hideki 名古屋大学, 大学院生命農学研究科, 准教授 (30314470)
• Research Collaborator: TAKAHARA Terunao 名古屋大学, 大学院生命農学研究科, 助教 (90708059) ; INOKAWA Akira 名古屋大学, 大学院生命農学研究科
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 脂肪前駆細胞 / エクソソーム / 細胞外膜小胞 / 転写因子

Outline of Final Research Achievements

We previously reported that phospholipid scramblase 3 (PLCSR3), stably expressed in human embryonic kidney (HEK) 293 cells, is secreted from the cells extracellularly as membrane vesicles (exosomes). In the present study, we clarified that PLSCR3 is expressed in mouse preadipocytic 3T3-L1 cells and that the amount of intracellular PLSCR3 decreased during differentiation but that of extracellularly secreted protein increased. Overexpression of PLSCR3 in 3T3-L1 cells suppressed adipocytic differentiation and transcription factor induction at the late stage. PLSCR3 was suggested to act as a negative regulator of adipogenesis.

Free Research Field

農芸化学・応用生物化学