2014 Fiscal Year Final Research Report
Studies on microbial fermentation heat production and its application
| Project/Area Number |
25660077
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied biochemistry
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Keywords | 発酵熱 / FoF1 ATPase / E. coli |
|---|
| Outline of Final Research Achievements |
We studied the mechanism of the fermentation heat production with the indigo leave fermentation as a model system. Several fungal species mainly contributed to the heat production, but some bacteria such as Pantoea dispersa and Bacillus subtilis were also involved in the process. Symplocarpus foetidus, a heat-producing plant, is known to produce heat by the ATP synthesis inhibition through the AOX-catalyzed oxidation of ubiquinone in the respiration chain. To examine if such an uncoupling-ATP synthesis also produces heat in bacteria, the heat production during the cell growth was compared among the wild type and FoF1 ATPase-mutant of E. coli strains, i.e., atpA and atpC. The heat production was assayed with Antares, a calorimeter designed for the bacterial cell growth monitoring. The heat production per cell by the FoF1ATPase-mutant cells was higher than that of the wild-type cells, which suggests that the inhibition of ATP synthesis causes the heat production with bacteria.
|
| Free Research Field |
応用生物化学
|
