Studies on the mechanoreception of the gastrointestinal cells in response to fluid shear stress produced by food hydrocolloids

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660105
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Food science
• Research Institution: Kyoto University
• Principal Investigator: TANI Fumito 京都大学, (連合)農学研究科(研究院), 教授 (70212040)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 物理的感覚受容 / ずり応力 / 血管内皮細胞 / 消化管上皮細胞 / カルシウム応答 / プリンヌクレオチド受容体 / P2X4 / 食品バイオレオロジー

Outline of Final Research Achievements

We analyzed the physiological responses of the gastrointestinal epithelial cells to a fluid shear stress. To complete the experimental design, we constructed a typical flow chamber system which is equipped with a micro-flow path under a microscopic observation, in which HEK293 cells overexpressed with P2X4 purinoreceptor were cultured on a slide glass at the bottom of the flow chamber, and the fluorescence of the transfectants can be detected with an oscillation of intracellular calcium in response to a fluid shear stress. Using this flow chamber system, we successfully observed that a fluid shear stress produced the calcium-induced fluorescence for both human colon adenocarcinoma HT29-MTX and pharyngeal carcinoma Detroit562 cell lines. Taken together the mRNA expression by quantitative RT-PCR for both types of cells, we suggest the possibility that the gastrointestinal epithelial cells can sense a fluid shear stress of luminal contents to regulate physiological functions of the gut.

Free Research Field

食品科学