How and why does the tiny nematode kill big pine trees?: Genetics-based approach to the pathogenic mechanism of pine wilt

2015 Fiscal Year Final Research Report

• Project/Area Number: 25660121
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Forest science
• Research Institution: Kyoto University
• Principal Investigator: Takeuchi Yuko 京都大学, (連合)農学研究科(研究院), 助教 (80452283)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: マツノザイセンチュウ / 組換え近交系 / RADシークエンス / ゲノムワイド関連解析

Outline of Final Research Achievements

The present study aimed to identify the pathogenic factor(s) of the pine wood nematode, Bursaphelenchus xyolophilus, the causal agent of pine wilt disease, with a genetic approach by using a set of recombinant inbred lines (RILs) which was newly-generated by repeating full-sib mating of two inbred lines. Results of phenotyping of RILs showed that the virulence and reproduction may be quantitative, polygenic trait and that transmission ability is a qualitative trait which is controlled by a single or few genes. Based on a genome-wide association analysis by use of a single nucleotide polymorphism (SNP) genotyping, several candidate genes of the pathogenic factor(s) such as signal peptidase gene and daf-1 gene were selected.

Free Research Field

樹木病理学