Development of bamboo flowering technology by direct protein transfer

2015 Fiscal Year Final Research Report

• Project/Area Number: 25660122
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Forest science
• Research Institution: Kyoto University
• Principal Investigator: Sakamoto Masahiro 京都大学, (連合)農学研究科(研究院), 准教授 (40303870)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 開花遺伝子 / タケ類 / タンパク質生産

Outline of Final Research Achievements

This study's object is the bamboo flowering by direct protein transfer. Introduced protein is FT, of which gene was already cloned from flowering dwarf bamboo (sasa) by me. cDNA of SvFT1 gene was introduced into the expression vector pGEX-6P-1, and its protein was produced in vitro. After production and purification of FT protein, this purified protein was introduced to dwarf bamboo through needle stabbed to the culm of dwarf bamboo. However, the culm area inserted needle resulted to necrosis, it did not lead to the flowering.

Free Research Field

樹木分子生物学