Development and application of sex-linked DNA markers in a natural population of a dioecious tree

2016 Fiscal Year Final Research Report

• Project/Area Number: 25660125
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Forest science
• Research Institution: Osaka City University
• Principal Investigator: NANAMI Satoshi 大阪市立大学, 大学院理学研究科, 准教授 (70326247)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 雌雄異株植物 / 性判別 / DNAマーカー / 性比 / 森林

Outline of Final Research Achievements

Five sex-linked DNA markers were developed in Nageia nagi (Podocarpaceae), a dioecious tree, using genome-wide RAD sequence data. We got 250,000 DNA sequence leads (100 bp) per individual in average. Although no female-specific sequences were found from these leads, 5 male-specific sequences were obtained. For the 5 sequences, PCR primer pairs were designed to identify sex of non-reproductive individuals.
For the assessment of sex ratio of non-reproductive seedlings, 148 seedlings from 4 sites in a natural population were analyzed on Mt. Mikasa, Nara Prefecture, Japan. The number of analyzed seedlings in a site raged from 32 to 45, and the proportion of males in a site varied from 0.465 to 0.600. No significant deviations of sex ratio from 1:1 were detected in any sites, and sex ratios were not significantly different among 4 sites. In the natural population of N. nagi, expected sex ratio at seedling stage was considered as 1:1 in any sites.

Free Research Field

植物生態学