2014 Fiscal Year Final Research Report
Novel technique for monitoring enzymatic hydrolysis of cellulose through high throughput DP analysis
| Project/Area Number |
25660138
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Wood science
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HORIKAWA Yoshiki 京都大学, 生存圏研究所, 特定研究員 (90637711)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | ケモメトリクス / 木質バイオマス / 分子量 / ハイスループット / セルロース / セルラーゼ / 近赤外分光分析 |
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| Outline of Final Research Achievements |
In order to improve saccharification performance, it is important to obtain precise information about functions and activities of individual enzymes in a cocktail of the cellulase. Particularly, endo-activity conventionally estimated by digestion of CMC, a model amorphous compound, did not often conform to the degradation of pretreated biomass. Since the accurate characterization of cellulose chain length seemed critical to the measure of endo-performance, highly dispersed microfibrillated cellulose samples were prepared as a standard substrate and the degree of polymerization (DP) was precisely monitored by GPC after tricarbanilation of cellulose. Surprisingly, for the first time, endo-activity of EG II was found to possess higher than that of EG I, while no considerable differences were found in those between CBH II and CBH I. Furthermore, facile and quick chemometrical monitoring system of DP was successfully developed using Near Infrared (NIR) spectra from the hydrolysates
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| Free Research Field |
セルロース科学 バイオマス科学
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