Can rainbow trout repeatedly produce gametes derived from iteroparous salmon?

2015 Fiscal Year Final Research Report

• Project/Area Number: 25660166
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Aquatic life science
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: YOSHIZAKI Goro 東京海洋大学, その他部局等, 教授 (70281003)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ニジマス / ヒメマス / 多回繁殖 / 一回繁殖 / 品種改良 / 生殖細胞移植

Outline of Final Research Achievements

Most Pacific salmon only spawn once. However, rainbow trout allows them to spawn multiple times. Since the ability to use iteroparous surrogate trout to produce semelparous salmon gametes would facilitate the production of semelparous salmon, we produced surrogate rainbow trout with germ cells derived from semelparous sockeye salmon. Seven males and three females out of twenty three recipients had matured by the second spawning season. Two of the seven males that had matured in the previous spawning season, matured again in the third spawning season. Further, one out of the three females matured again in the third spawning season, too. Compared to same-aged control rainbow trout, these recipient rainbow trout produced similar numbers of gametes. PCR-RFLP analyses showed that the banding patterns obtained for the offspring were identical to those of sockeye salmon. Taken together, we concluded that rainbow trout recipients could repeatedly produce sockeye salmon gametes.

Free Research Field

水族発生工学