Molecular mechanism of unique cell regeneration after wounding in polynuclear and unicellular marine green algae

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660168
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Aquatic life science
• Research Institution: Hiroshima University
• Principal Investigator: HORI KANJI 広島大学, 生物圏科学研究科, 教授 (50116662)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 海藻 / 再生 / 分子機構 / ハネモ

Outline of Final Research Achievements

Polynuclear and unicellular marine green algae possess unique regeneration ability because new cells are spontaneously formed from the cell contents extruded in seawater after wounding of algal tissues. Our previous studies suggested that the complex and/or aggregates of endogenous lectin proteins might involve in cell regeneration. In this study, three lectins (BPL54, BPL17 and recombinant BPL11) of Bryopsis plumosa were prepared and examined for clarifying the aggregation mechanism. The results showed that the formation of aggregates is not triggered by lectin-sugar interaction among the lectin molecules. rBPL11 did not show the organelle-aggregation ability even after heat-treatment of the protein, unlike BPL54 and BPL17. However, the mixture of BPL54, BPL17 and rBPL11 significantly caused the organelle-aggregation without heat-treatment. Thus, the endogenous lectin molecules appear to interact with each other and function in the unique cell regeneration after wounding.

Free Research Field

海洋生命科学