Electrical retrieval of intact and active symbiotic bacteria from deep-sea bivalves Calyptogena okutanii

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660173
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Aquatic life science
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: KOYAMA Sumihiro 独立行政法人海洋研究開発機構, 海洋生物多様性研究分野, 技術副主幹 (50344297)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 共生微生物 / 電気回収 / シロウリガイ / オオパンカイメン / ザラカイメン / バクテリア / アーキア

Outline of Final Research Achievements

We developed a novel electrical retrieval method for intact and active symbiotic bacteria from gill tissues of deep-sea bivalves Calyptogena okutanii and sponge-associated microorganisms from marine sponges. An indium tin oxide (ITO) optically transparent working electrode was placed on a chamber device with a counter- (Pt) and a reference (Ag/AgCl) electrode. We obtained 31 and 3 phyla of bacteria and archaea from C. okutanii and marine sponges S. insignis and Callyspongia confoederata. The electrically attached microorganisms had intact cell membranes and showed intracellular dehydrogenase activity. Dead microorganisms were not attracted to the electrode when the homogenized tissues were autoclaved before use.

Free Research Field

電極基板を利用した生きた微生物の電気回収