DNA-BASED RAPID BACTERIAL TESTING DEVICE IN PLANT FACTORY

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660203
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Agricultural environmental engineering/Agricultural information engineering
• Research Institution: The University of Tokyo
• Principal Investigator: TORII Toru 東京大学, 新領域創成科学研究科, 教授 (60172227)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: PCR / 遺伝子 / 微小流路 / 増幅効率 / 分注ロボット / ハイロドゲルモールディング法

Outline of Final Research Achievements

This research is development of Polymerase chain reaction (PCR) device in the microchannel. The device was fabricated using hydrogel molding method, and the dispenser robot was employed for the fabrication of complex geometry of microchannels. The conditions for the fabrication, such as heating and cooling temperature of gel, pressure of gel, moving speed of robot, dispensing position of the needle, was analyzed. The optimal conditions for the PCR was also analyzed, such as flow rate of reagents, concentration of polymerase, number of amplification, and the performance of the device was evaluated.

Free Research Field

微小流体工学