Function of microRNAs in bovine milk

2015 Fiscal Year Final Research Report

• Project/Area Number: 25660220
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Animal production science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Muroya Susumu 国立研究開発法人農業・食品産業技術総合研究機構, 畜産研究部門 畜産物研究領域, 上級研究員 (50355062)
• Co-Investigator(Kenkyū-buntansha): HAGI Tatsuro 国立研究開発法人農業・食品産業総合技術研究機構, 畜産研究部門・畜産物研究領域, 主任研究員 (00510257)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: マイクロRNA / ウシ / 牛乳 / 分泌

Outline of Final Research Achievements

Among major microRNAs (miRNAs) in a Holstein cow milk, expression of 18 miRNAs were analyzed in the bovine mammary epithelial cell (BMEC) culture that was treated with or without dexamethasone, insulin, and prolactin (DIP) to induce a lactogenic differentiation. Quantitative PCR results showed that the expression of miR-21-5p, miR-26a, and miR-320a was lower in the DIP-treated cells than in the untreated cells. In contrast, the expression of miR-339a in the cell culture medium were lower in the DIP-treated culture than in the untreated culture. Intriguingly, the miR-148a expression in cell culture medium was elevated by DIP treatment of BMEC culture. The medium-to-cell expression ratios of miR-103, miR-148a, and miR-223 were elevated in the DIP-treated BMECs. A bioinformatic analysis showed that the miRNAs down-regulated in the BMECs were associated with the suppression of genes related to transcriptional regulation, protein phosphorylation, and tube development.

Free Research Field

畜産、筋肉分子細胞生物