Identification of the important viral factor related to the development of rabies by the gene manipulation in mice

2015 Fiscal Year Final Research Report

• Project/Area Number: 25660225
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Gifu University
• Principal Investigator: Sugiyama Makoto 岐阜大学, 応用生物科学部, 教授 (80196774)
• Co-Investigator(Kenkyū-buntansha): ITO Naoto 岐阜大学, 応用生物科学部, 准教授 (20334922) ; Osawa Masatake 岐阜大学, 医学(系)研究科(研究院), 教授 (10344029)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 狂犬病ウイルス / 病態発生機序 / トランスジェニックマウス

Outline of Final Research Achievements

To identify rabies virus protein that is responsible for the pathogenesis, we tried to established transgenic (Tg) mice expressing each viral protein exclusively in neurons and then to reproduce rabies-like symptoms in the mice. In addition, we aimed to further examine this point by a combination use of the mice and the gene-deficient rabies viruses. Although we failed to generate L protein-expressing Tg mice, we established a plasmid required for G protein-expressing mice. Further, we have successfully generated L and G gene-deficient viruses before confirming their utilities.

Free Research Field

獣医学