Chemical hazard assessment with the genome-nuclear receptor interaction array system

2015 Fiscal Year Final Research Report

• Project/Area Number: 25660228
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Ehime University
• Principal Investigator: IWATA Hisato 愛媛大学, 沿岸環境科学研究センター, 教授 (10271652)
• Research Collaborator: YOSHINOUCHI Yuka 愛媛大学, 大学院理工学研究科博士前期課程, 学生 ; HIRANO Masashi 愛媛大学, 沿岸環境科学研究センター, 特任助教 ; KIM Eun-Young Kyung Hee University, Department of Biology, Associate Professor
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 核内受容体 / ビスフェノール類 / 水酸化ポリ塩化ビフェニル

Outline of Final Research Achievements

The aim of this study is to construct an in vitro assay system to screen hazardous environmental chemicals that can transactivate the gene via Baikal seal estrogen receptors (bsERs). We constructed the in vitro reporter gene assay system where a expression vector containing bsERα or bsERβ cDNA and a reporter vector containing ER response element were introduced in COS-1 cells. Using this in vitro assay system, we screened the transactivation potencies of 26 bisphenol A and its analogues (BPs) and 16 hydroxylated polychlorinated biphenyl congeners (OH-PCBs). The results showed that most of the test chemicals were able to activate bsERα and bsERβ.

Free Research Field

環境毒性学