Testis mediated gene transfer that a small laboratory can do the genetic modification in goat

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660239
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Gifu University
• Principal Investigator: TALASI Masaki 岐阜大学, 応用生物科学部, 准教授 (00503327)
• Co-Investigator(Kenkyū-buntansha): TAKASHIMA Yasuhiro 岐阜大学, 応用生物科学部, 准教授 (20333552)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: バイオリアクターヤギ / 遺伝子改変

Outline of Final Research Achievements

The aim of this study is to develop testis-mediated gene transfer that a small laboratory can do the genetic modification in goat.
In this study, we clarified 3 important fact: we developed isolation and short term culture of the testicular cells, DNA transfection into testicular cells is very difficult, and we obtained basic information about the cell transplantation technique into intact testis. Consequently, we found that the testis-mediated gene transfer in goat will be established when we keep researching on these.

Free Research Field

獣医臨床繁殖学