2014 Fiscal Year Final Research Report
A study on cartilage regeneration using intra-articular injection of synovial fluid and synovium derived mesenchymal stem cells.
| Project/Area Number |
25660242
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Veterinary medical science
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HOBO Seiji 鹿児島大学, 共同獣医学部, 教授 (60446507)
KAWAGUCHI Hiroaki 鹿児島大学, 共同獣医学部, 准教授 (60325777)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Keywords | 幹細胞 / 再生医療 / 骨 / 軟骨 / 関節 |
|---|
| Outline of Final Research Achievements |
We investigated the origin and the multipotency of mesenchymal stem cells (MSC) in the synovial fluid (SF) of horses with intra-articular injury and synovitis, and provided a new strategy for regeneration of lost articular cartilage. Total number of colonies of SF-MSCs from diseased joints was significantly higher than the number from normal joints. Under specific nutrient conditions, SF-MSCs differentiated into osteogenic, adipogenic and tenogenic lineages. Chondrogenic induction resulted in a formation of a gelatinous sheet, that was stained with Alcian blue. The results were consistent with those of synovium (SM)-derived MSCs. In the experimental model of pig which was injured osteocartilage in the shoulder joints, the intraarticular implantation (autologous transplantation) of SM-MSCs promoted the cartilage regeneration due to the adhesion of synovium to the damaged area. Allogenic implantation of equine SM-MSCs into the normal horse joint was safe and induced no side-effects.
|
| Free Research Field |
獣医外科学
|
