Clarification of molecular mechanism of selective follicular growth in mammals by identification of FSH binding inhibitor with different function

2015 Fiscal Year Final Research Report

• Project/Area Number: 25660247
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Integrative animal science
• Research Institution: National Livestock Breeding Center, Incorporated Administrative Agency
• Principal Investigator: Sato Eimei 独立行政法人家畜改良センター, その他部局等, 理事長 (80093243)
• Co-Investigator(Kenkyū-buntansha): HASHIYADA Yutaka (独)家畜改良センター, 企画調整部, 技術専門役 (50462536) ; MATSUDA Hideo (独)家畜改良センター, 企画調整部, 係長 (30442685) ; MASAKI Kyo (独)家畜改良センター, 企画調整部, 係長 (60731164)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 卵胞発育 / 血管増殖 / FAK / リン酸化 / IVMFC / 和牛

Outline of Final Research Achievements

The results obtained in this study are as follows. (1) Candidates of genes/proteins as the FSH binding inhibitor has been identified and some of them had the activity for GVBD Inhibition. (2) Hanging drop method for culturing individual oocyte-cumulus complex was established for screening the chemical including the inhibiutor for follicular development and oocyte maturation. Then, it was shown that the method is useful for our purpose. (3) The physiological role of FAK was analyzed, and it was shown that phosphorylation of FAK is important for oocytes to obtain the fertilizability and developmental ability through the expression the Has2. (4) It was shown that Pin1 has a crucial role for oocyte maturation and early embryogenesis in mice.

Free Research Field

家畜繁殖学、畜産学