2014 Fiscal Year Final Research Report
Optimization of direct reprograming methods by understanding the epigenomic features of descendant cell types.
Project/Area Number |
25660256
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Integrative animal science
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Research Institution | Keio University |
Principal Investigator |
ODA Mayumi 慶應義塾大学, 医学部, 助教 (80567511)
|
Research Collaborator |
SAKOTA Miki 慶應義塾大学, 医学部, 研究員
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Keywords | 直接リプログラミング / DNAメチル化 / 転写因子 / ES細胞 / エピゲノム |
Outline of Final Research Achievements |
In this study, we assessed the optimization of direct reprogramming method by analyzing the expression data of transcription factor (TF)-induced ES cell lines and found that the efficiency of cell differentiation examined by several cell type-specific markers was changed according to the role of TFs in direct reprogramming process. We also examined different culture conditions that alters genome-wide epigenetic status, and studied the results of TF-induction under different DNA methylation status. For the comparison, DNA methylation profiles of several tissues were made, which is expected to be used for the understanding the cell type-specific epigenomic patterns and the differences between their descendants.
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Free Research Field |
エピゲノム学
|