2015 Fiscal Year Final Research Report
The application of rabbit haploid embryonic stem cell to genome editing technology
| Project/Area Number |
25660259
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Kinki University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 半数体細胞 / ゲノム編集 / 胚性幹細胞 / ライブセルイメージング / ウサギ |
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| Outline of Final Research Achievements |
Haploid Embryonic stem cell(ESC) is more effective tool than conventional diploid ESC for genome editing. However, little is known about laboratory animal of haploid ESC besides the mouse. In this study, we aimed to obtain mouse and rabbit haploid ESC, and thus we tried to elucidate the characteristics of rabbit haploid embryo by live cell imaging technology. As a result, it showed to accumulation of developmental delays in each cell period of the produced rabbit haploid embryos are affecting the 8-cell stage or later development. On the other hand, we identified a new culture condition for inhibition of spontaneous polyploidy in haploid ESC. These findings indicated that haploid ESC has potential for new effective tool of genome editing in producing of human diseases animal model.
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| Free Research Field |
繁殖・発生工学
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