Basic research on development of functional-silk fiber using noncovalent immobilization strategy

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660269
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Insect science
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: ISHIBASHI Jun 独立行政法人農業生物資源研究所, 昆虫機能研究開発ユニット, 主任研究員 (20391576)
• Co-Investigator(Kenkyū-buntansha): IIZUKA Tetsuya (80414879)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: シルク / シルク結合ペプチド / 固定化 / 酵素 / 組換えカイコ / 機能性シルク / 固定化酵素

Outline of Final Research Achievements

Functional proteins were immobilized to silk fiber using a silk-binding peptide YN42 in transgenic silkworms. The immobilized proteins were stable on washing. The immobilized proteins were localized on the surface of silk fiber, suggesting highly reactive. The immobilized enzymes were active and durable. These results suggested the enzyme-immobilized silk fibers could be used for immobilized enzymes in industry.

Free Research Field

農芸化学