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2014 Fiscal Year Final Research Report

An interaction elucidation and its application of endobacteria present inside the hyphae of endophyte

Research Project

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Project/Area Number 25660273
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental agriculture(including landscape science)
Research InstitutionIbaraki University

Principal Investigator

NARISAWA Kazuhiko  茨城大学, 農学部, 教授 (90431650)

Co-Investigator(Kenkyū-buntansha) OHTA Hiroyuki  茨城大学, 農学部, 教授 (80168947)
SATO Yoshinori  独立行政法人国立文化財機構東京文化財研究所, 保存修復科学センター, 研究員 (50466645)
Project Period (FY) 2013-04-01 – 2015-03-31
Keywords共生 / 菌類内生バクテリア / エンドファイト
Outline of Final Research Achievements

Microscopic and molecular analyses showed the presence of endobacteria inside the hyphae of randomly selected fungal species. The 16S rRNA gene was successfully amplified with DNA extracted directly from the endobacterium-containing fungal isolates and most of the sequences were related to the family Burkholderiaceae.
Microscopic observation confirmed the presence of rod-shaped, motile bacteria living inside and outside the hyphae of Veronaeopsis simplex all isolates. The PCR results using universal primers for 16S sRNA positively detected the presence of bacteria on V. simplex. Analysis of terminal-restriction fragment length polymorphism (T-RFLP) coupled with the sequence data from 16S rDNA gene cloning revealed that Rhizobium sp. was found to be associated with the fungal isolates. In conclusion the findings suggest that V. simplex has a complex interaction with associated bacteria involving host plant growth promotion effect.

Free Research Field

農学

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Published: 2016-09-02  

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