Construction of chicken mutant library using primordial germ cells

2014 Fiscal Year Final Research Report

• Project/Area Number: 25660291
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Nagoya University
• Principal Investigator: IIJIMA Shinji 名古屋大学, 工学(系)研究科(研究院), 教授 (00168056)
• Research Collaborator: KANEOKA Hidenori 名古屋大学, 大学院工学研究科, 助教 (30631973)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: トランスジェニックニワトリ / 始原生殖細胞 / PRDM14 / 不均衡突然変異

Outline of Final Research Achievements

In order to establish chicken-mutant library, we tried to culture chicken primordial germ cells using STO cells expressing chicken LIF as feeder cells. By using this feeder cells, certain level of PGC-growth and expression of PGC-marker was observed but reproducibility was very low. Then, we analyzed a key regulator gene that governs and sustains PGC-differentiation and growth, in order to facilitate the PGC-culture experiments. We chose prdm 14 as a candidate, and cloned the gene. By the functional analyses, we found that prdm 14 up-regulated PGC-specific gene expression.

Free Research Field

生物工学