2014 Fiscal Year Final Research Report
Visualization of NADPH oxidase activity in plant immunity
| Project/Area Number |
25660293
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied molecular and cellular biology
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Keywords | バイオセンサー / 植物免疫 / 活性酸素 / NADPHオキシダーゼ |
|---|
| Outline of Final Research Achievements |
Reactive oxygen species (ROS) are important signaling molecules in plant immunity. Plant NADPH oxidase is termed as respiratory burst oxidase homologue (RBOH). Previously, we found that a calcium-dependent protein kinase (CDPK) activates RBOH by direct phosphorylation of the N-terminal region. Here, we tried to optimize a biosensor to patio-temporally monitor phosphorylation of the RBOH by CDPKs. However, we could not obtain fine construct of the sensor to generate enough intensity of fluorescence based on fluorescence resonance energy transfer (FRET).
|
| Free Research Field |
植物免疫学
|
