Synthesis of double labeled ceramide fluorescent probes to analyze metabolism of sphingolipids

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670003
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Chemical pharmacy
• Research Institution: Chiba University
• Principal Investigator: Nishida Atsushi 千葉大学, 薬学研究科(研究院), 教授 (80130029)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: セラミド / 代謝 / 蛍光標識 / NBD / BODIPY / ゴルジ

Outline of Final Research Achievements

Doubly labeled fluorescent probe at main and side chain on ceramide has been developed to obtain information of ceramide metabolism in cell. Such information will be useful to evaluate role of ceramide in connection with many diseases such as cancers. We developed first several green fluorescent ceramide probes. One of the ceramide probe which has NBD at main chain and an acety group on a primary hydroxy group was less toxic compared to commercially available C6-NBD ceramide and stain golgi system for a long period without damage on cell. We also developed a red fluorescent ceramide probe which has a BODIPY type group at main chain of ceramide. We also observed energy transfer between two florescent group such as NBD and BODIPY in the same ceramide molecule. Those results will be useful to develop a new fluorescent probes which is useful to analyze ceramide metabolism in living cells.

Free Research Field

有機合成化学