Development of HDL-like lipid membrane discs for reconstitution of membrane proteins

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670014
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: The University of Tokushima
• Principal Investigator: SAITO Hiroyuki 徳島大学, ヘルスバイオサイエンス研究部, 教授 (60300919)
• Co-Investigator(Kenkyū-buntansha): BABA Teruhiko 独立行政法人産業技術総合研究所, 幹細胞工学研究センター, 研究員 (40357794)
• Co-Investigator(Renkei-kenkyūsha): NAGAO Kohjiro 京都大学, 大学院工学研究科, 助教 (40587325) ; KAWAKAMI Toru 大阪大学, 蛋白質研究所, 准教授 (70273711)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: HDL / 脂質膜ディスク / アポA-I / ペプチド / 再構成 / 膜タンパク質

Outline of Final Research Achievements

In this study, we have developed novel HDL-like lipid membrane disc system based on (1) physicochemical basis of formation of HDL-like discoidal particles by apoA-I protein, (2) design and development of novel nanodisc scaffold peptides. We also performed (3) immobilization of lipid membrane discs reconstituted with apoA-I on a sensor chip and examination of real-time interaction with anti-apoA-I antibody. These results provide useful information to application of HDL-like lipid membrane discs to bioreactor/biosensor, single molecule structural analysis, and reconstitution of membrane proteins.

Free Research Field

生物物理化学