Development of tools and activity measurement methods for artificial manipulation of Notch signaling pathway

2016 Fiscal Year Final Research Report

• Project/Area Number: 25670020
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Chiba University
• Principal Investigator: ITOH MOTOYUKI 千葉大学, 大学院薬学研究院, 教授 (20377906)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: Notch / シグナル伝達 / 固相化

Outline of Final Research Achievements

Notch signaling plays important roles in various tissue development, and its manupiration is useful for artificially inducing the differentiation from pluripotent cells into target cells. In this study, human D114 and JAG1 ligand-immobilized beads were prepared and we demonstrated that they showed T cell-inducing activity from hematopoietic progenitor cells. Further, we succeeded in developing a Dll4 ligand protein with superior activity to wild type by introducing a point mutation.

Free Research Field

細胞生物学