2013 Fiscal Year Final Research Report
Analysis of YAP-dependent gene expression that regulates organ size.
| Project/Area Number |
25670021
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
NISHINA Hiroshi 東京医科歯科大学, 難治疾患研究所, 教授 (60212122)
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| Project Period (FY) |
2013-04-01 – 2014-03-31
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| Keywords | 器官サイズ / 転写 / シグナル伝達 / YAP / アセチル化 / リン酸化 |
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| Research Abstract |
Recently, YAP was shown to play an important role in organ size control and to be inhibited by the Hippo signaling pathway. Either transgenic overexpression of YAP or knockout of Hippo pathway genes in mouse liver results in enlargement of this organ and the eventual development of hepatic tumors. In this study, we used the method of hydrodynamic tail vein injection (HTVi) that is specific for gene transfer into liver. As a results, we succeeded in establishing an efficient system of liver cancer induction due to gain of function of the active YAP. Secondly, we analyzed the gene expression by cDNA microarray and identified more than 20 genes whose expression is increased by active YAP. Thus, our results could contribute to elucidate the mechanism of human liver cancer.
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[Journal Article] Control of the Hippo pathway by Set7-dependent methylation of Yap2013
Author(s)
Menno J. Oudhoff, Spencer A. Freeman, Amber L. Couzens, Frann Antignano, Ekaterina Kuznetsova, Paul H. Min, Jeffrey P. Northrop, Bernhard Lehnertz, Dalia Barsyte-Lovejoy, Masoud Vedadi, Cheryl H. Arrowsmith, Hiroshi Nishina, Michael R. Gold, Fabio M.V. Rossi, Anne-Claude Gingras, and Colby Zaph
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Journal Title
Dev. Cell
Volume: 26
Pages: 188-194
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