2014 Fiscal Year Final Research Report
Development of a system for stable expression of antibodies bearing non-natural amino acids at defined sites
Project/Area Number |
25670023
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Biological pharmacy
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Research Institution | Osaka University |
Principal Investigator |
HINO Nobumasa 大阪大学, 薬学研究科(研究院), 助教 (90469916)
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Project Period (FY) |
2013-04-01 – 2015-03-31
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Keywords | 拡張遺伝暗号 / 非天然型アミノ酸 / tRNA / 抗体薬物複合体 |
Outline of Final Research Achievements |
For stable production of antibodies with non-natural amino acids at specific positions, it is necessary to establish cell lines that constitutively and efficiently express exogenous amber suppressor tRNAs. To improve expression efficiency of the exogenous tRNAs, I attempted to develop a system that enables to express them from the nucleolus where cellular tRNAs are transcribed. I constructed tRNA expression vectors containing 5S rDNA sequences or genomic sequences surrounding cellular tRNA genes, which are expected to localize themselves to nucleolus. These vectors successfully increased the expression of the amber mutant of GFP by 1.5- to 2-fold as compared with the vector that contains tRNA genes alone.
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Free Research Field |
ケミカルバイオロジー
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