Identification of novel target proteins of NO with new screening system

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670029
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Okayama University
• Principal Investigator: UEHARA Takashi 岡山大学, 医歯(薬)学総合研究科, 教授 (00261321)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 一酸化窒素 / 翻訳後修飾 / スクリーニング系 / エピゲノム

Outline of Final Research Achievements

In this study, we attempted to isolate the novel target proteins of NO using new screening system. In addition, we tried to identify the substrates of NO with several specific antibodies. We could isolate several enzymes involved in epigenetics as the targets of NO. We found that those candidates are S-nitrosylated in vitro and in vivo. These results indicated that those proteins are certainly the targets of NO. We are now investigated the role of S-nitrosylation on its enzymatic activity.

Free Research Field

薬学系薬理