Study on the molecular mechanisms for interaction between mesenchymal stem cells and blood brain barrier using real-time imaging

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670033
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pharmacology in pharmacy
• Research Institution: Hokkaido University
• Principal Investigator: MINAMI Masabumi 北海道大学, 薬学研究科(研究院), 教授 (20243040)
• Co-Investigator(Kenkyū-buntansha): ISO Yukina ; MATSUBARA Akira ; MINAMISHIMA Takuya
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 神経科学 / 移植・再生医療 / 薬学

Outline of Final Research Achievements

We carried out antibody array analysis to identify the proteins contained in mouse astrocyte-conditioned medium (ACM), and examined the effects of those proteins on the blood-brain barrier (BBB) function using a mouse in vitro BBB model. We found that leukemia inhibitory facto (LIF) was contained in ACM and significantly attenuated barrier function. Because LIF belongs to Interleukin (IL)-6 family, we examined the effect of other family members IL-6 and oncostatin M on BBB function. Oncostatin M, but not IL-6, significantly attenuated barrier function. Further study for the molecular mechanism of the effects of LIF and oncostatin M may lead to development of the method to control BBB function.

Free Research Field

神経薬理学