Establishment of hypoxia-induced brain ischemia-reperfusion model in zebrafish larvae

2014 Fiscal Year Final Research Report

• Project/Area Number: 25670036
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pharmacology in pharmacy
• Research Institution: Kyoto University
• Principal Investigator: AKAIKE Akinori 京都大学, 薬学研究科(研究院), 教授 (80135558)
• Co-Investigator(Kenkyū-buntansha): IZUMI Yasuhiko 京都大学, 大学院薬学研究科, 助教 (60456837)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Keywords: 脳血管疾患 / ゼブラフィッシュ / 蛍光イメージング

Outline of Final Research Achievements

Although cerebrovascular disease is one of the major causes of death in Japan, the understanding of pathology and development of the therapeutic agents for this disease have been craving. In this study, we aim to develop new drug evaluation methods that contribute to the development of therapeutic drugs using zebrafish. Hypoxia-reoxygenation treatment induced ischemia-reperfusion in the brain of zebrafish larvae. Cell death in both head and trunk was induced by hypoxia-reoxygenation treatment. These results suggest that hypoxia-reoxygenation induces ischemia-reperfusion and cell death in zebrafish brain.

Free Research Field

神経薬理学