Plant alkaloid production system using fungi as host organism

2015 Fiscal Year Final Research Report

• Project/Area Number: 25670046
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Natural medicines
• Research Institution: Iwate Medical University
• Principal Investigator: Fujii Isao 岩手医科大学, 薬学部, 教授 (70181302)
• Co-Investigator(Kenkyū-buntansha): ASANO Takashi 岩手医科大学, 薬学部, 助教 (10552888) ; HASHIMOTO Makoto 岩手医科大学, 薬学部, 助教 (80552893)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: アルカロイド

Outline of Final Research Achievements

To utilize high productivity of secondary metabolites of fungi, construction of benzyl isoquinoline alkaloids biosynthesis system in Aspergillus oryzae was attempted. Expression plasmids of tyrsinase, monoamine oxidase, tyrosine decarboxylase, and norcoclaurine synthase genes were constructed. These plasmids were introduced into the host fungus A. oryzae by transformation. Analysis of induction culture products of transformants did not give any detectable benzyl isoquinoline alkaloids. Thus the direction of study was changed to isolate endophyte fungi producing alkaloids from alkaloid producing plants. So far some endophyte fungi were obtained from Amaryllidaceae plants.

Free Research Field

天然物化学