2014 Fiscal Year Final Research Report
in vivo biochemistry using optogenetics of cell signaling and FRET probe
| Project/Area Number |
25670088
|
|---|
| Research Category |
Grant-in-Aid for Challenging Exploratory Research
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General anatomy (including histology/embryology)
|
|---|
| Research Institution | Tokyo Medical and Dental University |
|---|
Principal Investigator |
NAKATA Takao 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (50218004)
|
|---|
| Project Period (FY) |
2013-04-01 – 2015-03-31
|
| Keywords | 光スイッチ / 解剖学 / 細胞組織 / シグナル伝達 / 神経科学 |
|---|
| Outline of Final Research Achievements |
The difference between cellls and chemical reaction in test tube was probably each reaction was properly organized in time and space. However,how the chemical reactions propagate in cells were still unknown. Actin is one of the most fundammental protein for cell motility and cytoarchitecture. However, its study on their regulatory mechanisms were not progressed for this 25 years, such as CDC42 and RAC1 polymerize actin filamnes of filopodia and lamelli podia. We use photoswitch of CDC42 and RAC1to regulate spatiotemporal regulation of these proteins, and monitor the cellular phenotypes using the FRET sensor and Life-act m Cherry markers,
|
| Free Research Field |
細胞生物学
|
