2014 Fiscal Year Final Research Report
An attempt to produce a transgenic medaka that express a cilia-related gene in a spatiotemporally regulated manner
| Project/Area Number |
25670098
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 発生 / 繊毛 / 嚢胞腎 |
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| Outline of Final Research Achievements |
I have attempted to produce a transgenic medaka that express a cilia-related gene in a spatiotemporally regulated manner by combination of Transcription Activator-Like Effector Nuclease (TALEN) method, Cre/loxP system and heat-shock promoter. I have already achieved that a TALEN knockout medaka in which a cilia-related gene in knocked out. This fish line developed a polycystic kidney as expected. Cystic dilation was already detected at hatching and gradually increased in size as fish grew. This phenotype resembles that of human polycystic kidney disease. Construction of a transgenic line using kidney specific promoter in progress.
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| Free Research Field |
発生
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