2014 Fiscal Year Final Research Report
The polarized localization of p62 and NBR1 in cathepsin D-deficient neurons is involved in selective autophagy
| Project/Area Number |
25670099
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | オートファゴソーム / p62 / NBR1 / リソソーム / ニューロン |
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| Outline of Final Research Achievements |
The present study revealed that p62/NBR1, adapter proteins of selective autophagy, localize in somatodendrites of neurons in cathepsin D (CD)-deficient mouse brains but not in axons. This localization pattern of p62 and NBR1 was confirmed in primary cultured cortical neurons, while the N-terminal specific domains of these proteins were responsible for the polarized localization. We further examined the intraneuronal target components of p62 and NBR1 and found that the target organelle was dysfunctional lysosomes in CD-deficient neurons. These results suggest that the polarized localization of p62 and NBR1 contributes to the selective autophagy in the somatodendrites of neurons.
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| Free Research Field |
解剖学一般（含組織学・発生学）
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