2014 Fiscal Year Final Research Report
Development of the DXT method by Photolithography
| Project/Area Number |
25670107
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | University of Fukui |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HIRAI Yoshikazu 京都大学, 大学院工学系研究科, 助教 (40452271)
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| Co-Investigator(Renkei-kenkyūsha) |
TABATA Osamu 京都大学, 大学院工学系研究科, 教授 (20288624)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | Single molecule / Ion channel protein / dynamics |
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| Outline of Final Research Achievements |
In this project we developed the Diffracted Xray Tracking(DXT) method,which can measure single-molecule conformational changes of ion channel proteins, by applying the photolithography technique to a probe and a recording chamber. In the DXT method a gold nanocrystal was attached to an ion channel protein as a probe, and the diffraction spots from the nanocrystal was elicited by radiation of the synchrotron white xray. Tracking the motions on the detector plane can be translated into the protein motions. We found the methods for fabrications of the size-controlled probe in nano-meter scale and the recording chamber with low xray-scattering noise in micrometer scale by using photolithography technique.
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| Free Research Field |
physiology
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