2014 Fiscal Year Final Research Report
Elucidation of molecular mechanism of nucleolar stress with a novel in vitro assay
| Project/Area Number |
25670136
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | 核小体 / リボソーム / RabL10 / RabL11 |
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| Outline of Final Research Achievements |
When cells meet stress such as nutrient deficiency, DNA damage, low oxygen condition or reactive oxygen species, they rapidly quit ribosomal biogenesiss and break the structure of nucleolus. This phenomenon is noted as the ‘nucleolar stresss’. The aim of this study is to elucidate the moleculer mechanism of the nucleolar stresss. We have tried to established a stable assay systems to evaluate it; a cell-free rRNA transcription assay with isolated nucleoli and a cell-free nucleolus break-down assay. We investigated the function of nucleolus-associated small GTPases rabL10 and RabL11, possible regulators for the nucleolar stress. We have identified their possible effector proteins and analyzed about the involvement.
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| Free Research Field |
biochemistry, cell biology
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