2014 Fiscal Year Final Research Report
Screen to identify an osteoblast membrane receptor and its functional analysis
| Project/Area Number |
25670142
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
General medical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Keywords | Obif / 骨芽細胞 / 軟骨細胞 / 骨粗鬆症 |
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| Outline of Final Research Achievements |
Osteoporosis is one of the most important theme to be solved in a rapidly aging society. We screened genes regulating chondrocyte differentiation, and identified Obif, a single transmembrane protein, and Cfm2, which is highly expressed in proliferating and prehypertrophic chondrocytes. We generated Cfm1/Cfm2 double-knockout (Cfm DKO) mice. The number of cartilaginous cells in intervertebral discs is remarkably reduced, and chondrocytes are moderately reduced in Cfm DKO mice. In addition to interaction between Cfm and Filamin proteins, we showed that Cfm is required for the interaction between Flnb and Smad3. Multiple lines of evidence reported mutational congenital anomalies of Flnb including autosomal recessive spondylocarpotarsal syndrome, autosomal dominant boomerang dysplasia, Larsen syndrome, and atelosteogenesis I and III phenotypes. This study is expected to accelerate the development of novel diagnosis and cure methods for congenital skeletal malformation in the future.
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| Free Research Field |
神経発生学
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